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91.
92.
Abstract 1. When offered a choice, female diamondback moths (Plutella xylostella) oviposited more eggs on plants with non‐parasitised conspecific larvae than on plants with parasitised larvae. 2. The leaf area consumed by parasitised larvae was significantly lower than that by non‐parasitised larvae. However, this quantitative difference in larval damage did not explain the female’s ability to discriminate between plants with parasitised and non‐parasitised larvae, as females showed an equal oviposition preference for plants infested by higher or lower densities of non‐parasitised larvae. 3. Pupal weight and duration of the larval stage of P. xylostella were independent of whether larvae were reared on plants that were previously infested by either non‐parasitised or parasitised larvae. 4. The larval parasitoid Cotesia vestalis did not distinguish between plants infested by non‐parasitised larvae and plants infested by larvae that had already been parasitised by conspecific wasps. 5. Based on these data, it can be concluded that the moth oviposition preference for plants infested by non‐parasitised conspecifics relative to plants infested by parasitised conspecifics was not explained by plant quality or by the attractiveness of plants towards wasps. It is hypothesised that one of the reasons for this preference is avoidance of plants where a relatively high risk of parasitism is expected due to the emergence of parasitoids from the parasitised host larvae. 相似文献
93.
Phillip A. Wilmarth Michael A. Riviere Larry L. David 《Journal of ocular biology, diseases, and informatics》2009,2(4):223-234
Analysis of shotgun proteomics datasets requires techniques to distinguish correct peptide identifications from incorrect identifications, such as linear discriminant functions and target/decoy protein databases. We report an efficient, flexible proteomic analysis workflow pipeline that implements these techniques to control both peptide and protein false discovery rates. We demonstrate its performance by analyzing two-dimensional liquid chromatography separations of lens proteins from human, mouse, bovine, and chicken lenses. We compared the use of International Protein Index databases to UniProt databases and no-enzyme SEQUEST searches to tryptic searches. Sequences present in the International Protein Index databases allowed detection of several novel crystallins. An alternate start codon isoform of βA4 was found in human lens. The minor crystallin γN was detected for the first time in bovine and chicken lenses. Chicken γS was identified and is the first member of the γ-crystallin family observed in avian lenses. 相似文献
94.
Navin Elango Jeeyoung Lee Zuogang Peng Yong-Hwee E. Loh Soojin V. Yi 《Biology letters》2009,5(3):405-408
We analysed over 8 million base pairs of bacterial artificial chromosome-based sequence alignments of four Old World monkeys and the human genome. Our findings are as follows. (i) Genomic divergences among several Old World monkeys mirror those between well-studied hominoids. (ii) The X-chromosome evolves slower than autosomes, in accord with ‘male-driven evolution’. However, the degree of male mutation bias is lower in Old World monkeys than in hominoids. (iii) Evolutionary rates vary significantly between lineages. The baboon branch shows a particularly slow molecular evolution. Thus, lineage-specific evolutionary rate variation is a common theme of primate genome evolution. (iv) In contrast to the overall pattern, mutations originating from DNA methylation exhibit little variation between lineages. Our study illustrates the potential of primates as a model system to investigate genome evolution, in particular to elucidate molecular mechanisms of substitution rate variation. 相似文献
95.
To assess the potential of short-term screenings for drought resistance at the seedling stage to detect ecotypic variation
and predict field performance, we studied the responses to water deficit of seedlings of Pinus canariensis from five geographic origins under controlled conditions and compared these responses with the performance of provenances
in a multi-site field trial. Leaf water potential, the osmotic component, leaf chlorophyll fluorescence and growth and biomass
partitioning were measured as seedlings were subjected for 11 days to two levels of osmotic potential generated by polyethylene
glycol (PEG 6000), −1 MPa (slowly imposed water deficit; S) and −1.5 MPa (fast imposed water deficit; F), and a control treatment
(no PEG added to the nutrient solution; C). Leaf water potential declined to final mean values of −1.2, −2.7 and −4.7 MPa
in the C, S and F treatments, respectively. The ratio of variable to maximum chlorophyll fluorescence declined to final mean
values of 0.77, 0.66 and 0.40 in the C, S and F treatments, respectively, with no differences amongst provenances. All provenances
showed an active osmotic adjustment (OA) in response to water deficit which varied depending on the drying rate. A slow imposition
of water deficit favoured solute accumulation. Pooling all treatments, the index of OA ranged from 0.28 to 0.40, but rose
considerably when only C and S treatments were considered (0.56 to 0.70). There was a positive and significant correlation
between the overall index of OA (all treatments pooled) and the drought period in the site of origin, suggesting ecotypic
variation in OA as a result of drought duration. Seedlings allocated more dry matter to roots than shoots when subjected to
moderate and slowly imposed water deficit; only one provenance showed no increase in the root to shoot ratio at the end of
the treatment period compared with control seedlings. Responses to controlled water deficits were only qualitatively related
to performance (survival and growth) of provenances in several field sites, indicating the involvement of complex mechanisms
to cope with drought under natural conditions. However, the provenance with the highest overall index of OA outgrew and outsurvived
the other provenances in the most arid site, and the only provenance not modifying the root to shoot ratio in response to
water deficit survived the least in all field sites. Acclimation of root to shoot ratio and net solute accumulation to water
deficit could hence favour drought-tolerance beyond the seedling stage and be used as preliminary predictors of field performance. 相似文献
96.
Ocen M. Olanya Andrew B. Plant Robert P. Larkin Charles W. Honeycutt 《Journal of Phytopathology》2009,157(7-8):427-437
Infection of hairy nightshade ( Solanum sarrachoides Sendt.) by Phytophthora infestans has been reported. However, the epidemiological significance of hairy nightshade to potato late blight is not well known. Disease development and infection rates of P. infestans were quantified on hairy nightshade relative to tomato (cv. Brandywine) and potato (cv. Shepody) hosts to evaluate infection potential at 14, 18, 22 and 26°C and 72, 82, 87, and 92% relative humidity (RH). The susceptibility of hairy nightshade to inoculum levels, weed ontogeny, and sporangia production potential were also investigated. Late blight development varied among hairy nightshade, tomato and potato hosts. Pathogen infection rates ranged from 0.0325 to 0.4674 gompits/day (unit for quantifying infection rates), and were significantly (P < 0.05) greater on potato and tomato than on hairy nightshade. Late blight severity was variably affected by RH. Disease levels on hairy nightshade varied with inoculum load; and ranged from 9 to 26% and 26 to 37% at low (5 × 103 ) and high (25 × 103 ) sporangia concentrations, respectively. Late blight was recorded irrespective of hairy nightshade ontogeny, and was significantly greater on 8–10 than 4–6-week-old plants. These results indicate that pathogen, environmental and host factors affect late blight development on hairy nightshade. 相似文献
97.
We investigated the variation of Antarctic fur seal (Arctocephalus gazella) pup growth rates in response to sex, breeding season and duration of both maternal foraging trip and attendance bouts. Data
were collected during five consecutive rearing seasons at Cape Shirreff, the most important breeding colony in the South Shetland
Islands, Antarctica. Our results showed significant interannual and sexual variations in pup growth rates. Male pups grew
significantly faster than female pups during 2000, 2001, 2002 and 2004 seasons, whilst during 2003 no difference was found.
The interannual variation in pup growth rates was correlated with the interannual fluctuation in maternal foraging trip and
attendance bouts. There was a significant effect of pup sex and maternal foraging trip duration on pup growth rates, which
varied between years having foraging trip duration a major effect during 2003, when females spent more time at sea and interestingly
on that year there were no sexual differences in pup growth rates. The effect of attendance bout on pup growth rates was not
significant. Diet analysis showed that Antarctic krill (Euphausia superba) was the most frequent prey item during the study period. Analysis of krill size distribution showed a significant difference
in krill length, during 2003, when A. gazella preyed upon the smallest sizes of krill. In this study, sex was the most important factor on pup growth rates, but when prey
availability seemed more limited, there are longer foraging trips and shorter attendance bouts the sex factor became less
significant. 相似文献
98.
The coldwater crayfish Orconectes eupunctus is endemic to the Spring and Eleven Point Rivers of Arkansas and Missouri, and appears to have been displaced from a portion
of its range by the recently introduced ringed crayfish Orconectes neglectus. We examined competition among juveniles as a potential mechanism for this crayfish species displacement through laboratory
and field experiments. Orconectes eupunctus juveniles survived and grew in stream cages in their former range, implicating biotic interactions rather than habitat degradation
in the displacement. Laboratory experiments revealed O. neglectus juveniles were dominant in the presence of limited food, whereas size rather than species determined occupancy of limited
shelter. In a field competition experiment using stream cages, O. neglectus juveniles did not inhibit growth or reduce survival of O. eupunctus juveniles. Consequently, laboratory evidence of O. neglectus dominance did not correspond with competition under field conditions. Combined with previous studies examining the effects
of O. neglectus on O. eupunctus, these results suggest that competition may not be a factor in this crayfish species displacement. Alternate mechanisms for
the apparent displacement of O. eupunctus by O. neglectus, such as differential predation or reproductive interference, should be investigated. 相似文献
99.
The selection of a sampling protocol is critical to study amphibian and reptile communities and in many instances researchers have combined the use of visual encounter surveys (VES) conducted on trails and off trails. The effect of human‐made trails on relative abundance estimates of amphibians and reptiles has been assessed in a few temperate locations, but data are lacking for tropical sites. Our study was designed to address this issue by comparing abundance estimates of frogs and lizards on and off trails in a lowland rainforest in south‐eastern Perú. We used nocturnal VES to sample frogs and lizards along transects established on trails and off trails in two different forest types. We found that the observed relative abundance estimates of frogs and lizards were affected by the location of transects (on trail vs. off trail) and the type of forest (floodplain forest vs. terra firme forest). We also found an interaction between the two main effects, indicating that the effect of transect location with respect to trails varies as a function of habitat. Observed frog abundances were higher on trails than off trails, indicating that studies that include VES on trails will bias relative abundance estimates in contrast to studies that include only VES off trails. We suggest that transects should be established only off trails, especially for monitoring studies because trail use by humans can have a strong influence on observed animal abundance. 相似文献
100.
Male-biased infection is a common phenomenon in vertebrate-parasite systems and male-biased transmission has been experimentally demonstrated. One mechanism that is hypothesized to create male-biased transmission is the immuno-suppressive effect of testosterone because it increases susceptibility to infection. Testosterone also influences host behaviour and, consequently, may increase exposure to parasites. To test how testosterone could increase exposure and transmission, we undertook a longitudinal mark-recapture study where we experimentally elevated testosterone levels in wild male rodents. Individuals in control populations reduced the average number of contacts over the treatment period, while populations with experimentally elevated testosterone levels maintained the number of contacts between hosts. As a result, the transmission potential was higher in testosterone treated populations compared to controls. Our results indicated that males with high-testosterone levels alter the population-level contacts, producing different social networks and increasing transmission potential compared to those where testosterone is at background levels. 相似文献